This presentation was given on July 21, 2021, sponsored by the Common Fund Data Ecosystem. The presentation was made by Dr. Amanda Charbonneau. This two-hour workshop presents a coding-free overview of RNA sequence analysis with a focus on differential expression. Experimental design and sample preparation are discussed and analysis workflows are explored. By the end of this workshop, you will know the fundamentals of designing a differential expression experiment, have a conceptual understanding of the process of RNA-Seq analysis, and understand common RNA-Seq problems and how to prevent them.